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In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin

JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze

In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin

In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin

DOI:

10.3791/51045-v

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07:31 min

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August 24, 2014

•

Mikhail Yuryev, Dmitry Molotkov

¹Neuroscience Center,University of Helsinki

Capitoli

00:00Titolo
01:03Mouse Preparation and Skin Fixation
03:11Imaging
03:47Laser Lesion
04:52Data Analysis and Representative Results
06:30Conclusions

Summary

Traduzione automatica

The protocol for dynamic longitudinal imaging and selective laser lesion of nerve endings in reporter transgenic mice is presented.

Tags

Keywords: In Vivo Two-photon Microscopy Nerve Endings Skin Reporter Transgenic Mice EYFP Thy-1 Longitudinal Studies Laser-based Microsurgery

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