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Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
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JoVE Journal Biologia
Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

DOI:
10.3791/51058-v

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10:30 min

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January 20, 2014

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, ,
1Department of Cell and Developmental Biology,Vanderbilt University Medical Center

Capitoli

  • 00:05Titolo
  • 01:30Testes Dissection
  • 03:13Sample Preparation and Live Imaging
  • 04:49Formaldehyde Fixation and Antibody Staining
  • 07:49Results: Microscopic Images of Drosophila Testes
  • 09:57Conclusion

Summary

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Traduzione automatica

Methods for isolating and preparing Drosophila testes samples (live and fixed) for imaging by phase-contrast and fluorescence microscopy are described herein. 

Tags

DrosophilaSpermatogenesisTestesCytological AnalysisLive PreparationFixed PreparationPhase-contrast MicroscopyFluorescence MicroscopyMutant CharacterizationProtein Localization

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