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सक्रिय कस्पासे 3 का पता लगाने के लिए पूरे माउंट immunofluorescence द्वारा zebrafish भ्रूण में apoptosis के विश्लेषण
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
DOI:

08:20 min

December 20, 2013

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Capitoli

  • 00:05Titolo
  • 01:24Preparation of Solutions and Embryos
  • 03:17Immunofluorescence Staining and Microscopy
  • 05:43Representative Results
  • 07:56Conclusion

Summary

Traduzione automatica

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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