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小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移
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Neuroscienze
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JoVE Journal Neuroscienze
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
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小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移

DOI:
10.3791/51070-v

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09:07 min

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March 17, 2014

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, , ,
1Cellular and Molelcular Neurobiology,Max Planck Institute of Experimental Medicine, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Capitoli

  • 00:05Titolo
  • 01:26Transfection of Cultured Neurons
  • 04:20In Vivo Electroporation
  • 07:10Results: Genetic Manipulation of Cerebellar Granule Neurons to Study Neuronal Morphology and Migration
  • 08:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

神经元的形态发生和迁移的基本正常的大脑发育至关重要的事件。在这里，我们描述的方法来操纵基因培养的小脑颗粒神经元和小脑发展的形态和神经元的迁徙特性的评估。

Tags

Cerebellar Granule NeuronsGenetic ManipulationNeuronal MorphogenesisNeuronal MigrationIn Vitro AnalysisIn Vivo AnalysisAxonogenesisDendritogenesisRNA InterferenceOverexpressionSmall MoleculesRodent Cerebellar CortexIn Vivo Electroporation

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