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人体前庭神经鞘瘤的原代培养
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Medicina
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JoVE Journal Medicina
Primary Culture of Human Vestibular Schwannomas
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人体前庭神经鞘瘤的原代培养

DOI:
10.3791/51093-v

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10:50 min

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July 20, 2014

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, ,
1Department of Otolaryngology-Head and Neck,University of Iowa Hospitals and Clinics

Capitoli

  • 00:05Titolo
  • 01:31Setup: The Day Before Tumor Harvest
  • 02:21Setup and Tumor Harvest
  • 04:08Tissue Dissociation and Trituration
  • 07:59Cell Plating
  • 09:19Results: Primary Vestibular Schwannoma Cultures
  • 10:18Conclusion

Summary

Traduzione automatica

Traduzione automatica

前庭神经鞘瘤（VSS）是雪旺细胞（SC）的原点非恶性肿瘤，与在NF2的肿瘤抑制基因的突变相关联。我们提出一个可重复的，高效的协议，用于原代人类VS细胞培养，允许分子和细胞实验操作和分析，概括人类疾病的异质性。

Tags

Vestibular SchwannomaSchwann Cell TumorNeurofibromatosis Type 2NF2 Tumor Suppressor GenePrimary Cell CultureHEI193 Cell LineHPV E6 And E7 OncogenesTumorigenesis

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