Rivista
/
/
Visualisatie van de immunologische Synapse door Dual Color Time-gated stimulated emission depletion (STED) Nanoscopy
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
DOI:

10:00 min

March 24, 2014

,

Capitoli

  • 00:05Titolo
  • 01:25Activation of NK Cell on Antibody-coated Coverslips
  • 03:19Fixing, Permeabilizing, and Staining NK Cells on Coverslips
  • 04:52Mounting the Coverslips
  • 05:35STED Imaging
  • 08:21Optimizing STED Imaging: F-actin in NK Cells
  • 09:30Conclusion

Summary

Traduzione automatica

Hier illustreren we het protocol voor beeldvorming door twee kleuren STED nanoscopy de cytotoxische immuun synaps van NK-cellen samengevat op glas. Met deze methode krijgen we sub-100 nm resolutie van synapseiwitten en het cytoskelet.

Video correlati

Read Article