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原位胶质母细胞瘤小鼠模型的维护脑实质的物理约束，并适用于活体双光子显微镜

JoVE Journal
Medicina

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JoVE Journal Medicina

An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy

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原位胶质母细胞瘤小鼠模型的维护脑实质的物理约束，并适用于活体双光子显微镜

DOI:

10.3791/51108-v

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09:52 min

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April 21, 2014

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Clément Ricard², Fabio Stanchi³, Geneviève Rougon², Franck Debarbieux²

¹Developmental Biology Institute of Marseille,Aix Marseille University, ²European Research Center for Medical Imaging, Campus de la Timone, ³Vesalius Research Center,KU Leuven Campus Gasthuisberg

Capitoli

00:05Titolo
01:26Craniotomy
03:48Spheroid Preparation and Implantation
04:58Closing the Craniotomy with a Window
07:59Tissue Imaging
09:20Conclusion

Summary

Traduzione automatica

我们已经建立了原位皮质胶质母细胞瘤模型小鼠的活体双光子显微镜的概括了生物物理的限制，通常在作怪肿瘤的生长过程。一种慢性玻璃窗替换上面的肿瘤颅骨使后续的肿瘤进展时间的推移由双光子显微镜。

Tags

Glioblastoma Mouse Model Orthotopic Two-photon Microscopy Brain Parenchyma Tumor Spheroid Dextran Gel Craniotomy Fluorescent Transgenic Intravital Imaging Neuro-oncology Drug Testing

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