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Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice
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Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice

Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice

DOI:
10.3791/51110-v

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10:16 min

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December 26, 2013

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, ,
1Department of Molecular & Cellular Physiology,Louisiana State University Health Sciences Center

Capitoli

  • 00:05Titolo
  • 01:25Preparation of Perfusion Solutions
  • 02:16Animal Anesthesia and Vascular Cannulation
  • 03:45Preparation for Intravital Microscopy
  • 04:41Infusion of Fluorescent Microspheres to Measure Velocities
  • 05:15Infusion of a Fluorescent Plasma Marker to Measure Diameters
  • 06:11Video Analysis
  • 07:56Results: Intravital Microscopy Measurements of Retinal Blood Flow
  • 09:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

Intravital microscopy can be used in animals to visualize and measure retinal vascular diameters, bloodstream velocities, and total retinal blood flow.

Tags

Keywords: Intravital Video MicroscopyRetinal Blood FlowMiceFluorescent TracerArterioleVenuleCentral Retinal ArteryCentral Retinal VeinVascular DiameterBloodstream VelocityRetinal PerfusionOcular DiseaseVisual Dysfunction

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