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Dissektion af<em> Xenopus laevis</em> Neural Crest<em> In vitro</em> Eksplantat kultur eller<em> In vivo</em> Transplantation
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Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Dissektion af<em> Xenopus laevis</em> Neural Crest<em> In vitro</em> Eksplantat kultur eller<em> In vivo</em> Transplantation

DOI:
10.3791/51118-v

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09:07 min

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March 04, 2014

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,
1Institut Curie,Centre Universitaire, 2Université Paris Sud,Centre Universitaire, 3CNRS UMR 3347,Centre Universitaire, 4INSERM U1021,Centre Universitaire

Chapters

  • 00:05Title
  • 01:19Dissection of the neural Crest (NC) Explants
  • 03:54Plating Explants on Fibronectin for in vitro Migration Imaging
  • 05:03Grafting Explants into Host Embryos for in vivo Migration Analysis
  • 06:25Results: In vitro and in vivo Behavior of Explanted Cranial NC
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver, hvordan man dissekere premigratory kraniel neuralkam (NC) fra Xenopus laevis neurulas. Disse eksplantater kan udpladet på fibronectin og dyrket in vitro, eller podes tilbage i værten embryoner. Denne teknik gør det muligt at studere de mekanismer NC epitel-til-mesenchyme overgang, migration og differentiering.

Tags

Neural CrestXenopus LaevisDissectionExplant CultureIn Vivo TransplantationEpithelium-to-mesenchyme TransitionMigrationDifferentiation

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