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Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts
DOI:

08:15 min

February 11, 2014

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Capitoli

  • 00:05Titolo
  • 01:37Preparation of Cell Lines
  • 02:54Preparation of Animals and Experimental Setup
  • 04:14Separation of Bladder Wall Layers and Intramural Inoculation of Bladder cancer Cells
  • 05:54Representative Bladder Cancer Injection Results
  • 07:39Conclusion

Summary

Traduzione automatica

The established technique to inoculate primary invasive orthotopic bladder cancer xenografts requires laparotomy and mobilization of the bladder. This procedure inflicts significant morbidity on the mice, is technically challenging and time-consuming. We therefore developed a high-precision, percutaneous approach utilizing ultrasound guidance.

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