/
/
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Biophysical Analytics, German Rheumatism Research Center,Leibniz Institute, 2Microscopy Core Facility,Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center,Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine
Capitoli
- 00:05Titolo
- 01:26Multi-beam Setup for Striped-illumination TPLSM
- 06:07Results: Highly-resolved Intravital Striped-illumination Images
- 09:05Conclusion
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
