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快速合成及其与绿色荧光蛋白为基础的记者化学激活的转录因子筛选
JoVE Journal
Biologia
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JoVE Journal Biologia
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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快速合成及其与绿色荧光蛋白为基础的记者化学激活的转录因子筛选

DOI:
10.3791/51153-v

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09:22 min

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November 26, 2013

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, , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Department of Molecular Biology,Princeton University, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Capitoli

  • 00:05Titolo
  • 01:33Creation of Artificial Transcription Factors (ATFs)
  • 02:28Creation of ATF Plasmid Reporter
  • 03:57Quantify the Effect of ATF Induction on Gene Expression Using GFP Reporter
  • 05:33Quantify the Effect of the ATF Induction on Cell Growth
  • 06:42Create Inducible Genomic Alleles
  • 07:20Results: Analysis of GFP Induction by ATF-promoter Pairs
  • 08:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

本协议描述了实验过程的人工转录因子（申请进度查询设施）与绿色荧光蛋白同源记者和通过流式细胞仪，刺激GFP表达的申请进度查询设施能力量化的快速建设。

Tags

Synthetic BiologyTranscription FactorsGene ExpressionReporter SystemsGFPHomologous RecombinationArtificial Transcription FactorsConditional Gene Expression

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