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自下而上和猎枪蛋白质组学鉴定一个全面的蛋白质组耳蜗
JoVE Journal
Biologia
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JoVE Journal Biologia
Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
DOI:

14:23 min

March 07, 2014

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Capitoli

  • 00:05Titolo
  • 02:13Protein Extraction
  • 03:03Endoproteinase LysC and Tryptic Protein Digestion of Whole Lysate Using Filter Assisted Sample Preparation
  • 05:34Ion Exchange Chromatography
  • 06:20GELFREE Fractionation of Cochlear Sensory Epithelium
  • 07:54Protein Digestion of GELFREE Fractions Using Filter Assisted Sample Preparation
  • 10:07Results: Identification of membrane and Soluble Proteins by High-resolution Mass Spectrometry Analysis
  • 14:00Conclusion

Summary

Traduzione automatica

耳蜗感觉上皮蛋白质组分析可能是由于其体积小和具有挑战性的,因为膜蛋白很难分离和鉴定。两膜和可溶性蛋白质可以通过组合多个的制备方法和分离技术随着高分辨率质谱来识别。

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