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Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
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JoVE Journal Immunologia e infezioni
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

DOI:
10.3791/51189-v

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13:34 min

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March 17, 2014

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,
1Department of Immunology,The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences

Capitoli

  • 00:05Titolo
  • 01:56Hydrodynamic Gene Transfer
  • 02:54Adoptive Transfer of Common Dendritic Progenitors (CDPs)
  • 06:38Isolation and Enumeration of Peyer’s Patch Plasmacytoid Dendritic Cells
  • 09:36Results: Analysis of PP pDC in Ifnar/- Mice Upon Flt3L HGT and Adoptive Transfer of CPDs
  • 13:12Conclusion

Summary

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This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.

Tags

Keywords: Murine Plasmacytoid Dendritic CellsPeyer’s PatchesAdoptive TransferHematopoietic ProgenitorsCommon Dendritic Cell ProgenitorsFlt3 LigandIfnar-/- MiceFlow Cytometry

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