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评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

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评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移

DOI:

10.3791/51189-v

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13:34 min

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March 17, 2014

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Haiyan S. Li, Stephanie S. Watowich²

¹Department of Immunology,The University of Texas MD Anderson Cancer Center, ²The University of Texas Graduate School of Biomedical Sciences

Capitoli

00:05Titolo
01:56Hydrodynamic Gene Transfer
02:54Adoptive Transfer of Common Dendritic Progenitors (CDPs)
06:38Isolation and Enumeration of Peyer’s Patch Plasmacytoid Dendritic Cells
09:36Results: Analysis of PP pDC in Ifnar^/- Mice Upon Flt3L HGT and Adoptive Transfer of CPDs
13:12Conclusion

Summary

Traduzione automatica

这个协议描述的实验程序来评估浆细胞样树突细胞的Peyer氏从共同的树突状细胞前体细胞的补丁的分化，使用涉及FACS介导的细胞分离，水动力的基因转移，并在淋巴集结免疫子集流分析技术。

Tags

Keywords: Murine Plasmacytoid Dendritic Cells Peyer’s Patches Adoptive Transfer Hematopoietic Progenitors Common Dendritic Cell Progenitors Flt3 Ligand Ifnar-/- Mice Flow Cytometry

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