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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Double Negative αβ T Cells from the Kidney
 

Isolation of Double Negative αβ T Cells from the Kidney

Article DOI: 10.3791/51192-v • 06:56 min • May 16th, 2014
May 16th, 2014 •
1, 2, 2, 1
1Department of Pathology, Johns Hopkins University School of Medicine, 2Department of Medicine, Johns Hopkins University School of Medicine

Capitoli

Riepilogo
Automatic Translation

DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.

Tags

Keywords: Double Negative T Cells DN T Cells Kidney Isolation Protocol CD45+ Selection Negative Selection CD4 CD8 MHC Class II CD1d α-galcer Tetramer Purity
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