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Anti-nukleare Antibody Screening Brug HEp-2-celler
JoVE Journal
Biologia
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JoVE Journal Biologia
Anti-Nuclear Antibody Screening Using HEp-2 Cells

Anti-nukleare Antibody Screening Brug HEp-2-celler

DOI:
10.3791/51211-v

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13:01 min

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June 23, 2014

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, , ,
1IFA Development Manager,INOVA Diagnostics, Inc., 2IFA Development,INOVA Diagnostics, Inc., 3Product Manager, Rheumatology,INOVA Diagnostics, Inc., 4Medical Director,INOVA Diagnostics, Inc.

Capitoli

  • 00:05Titolo
  • 02:11Sample Preparation and Substrate Selection
  • 03:08Addition of Controls and Samples
  • 04:34Addition of Fluorescent Conjugate
  • 06:08Identification of Positive and Negative Results
  • 07:52Results: Cell Staining Patterns for HEp-2
  • 11:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

Indirekte immunofluorescens (IIF) assays er traditionelt blevet anvendt til påvisning af antinukleare antistoffer (ANA) i humant serum. Tilstedeværelsen af ​​disse antistoffer kan støtte i diagnosticering af systemiske autoimmune reumatiske sygdomme (SARD). Denne protokol viser, hvordan man effektivt udfører IIF teknik til præcist at opdage disse autoantistoffer.

Tags

Anti-nuclear AntibodyANAHEp-2 CellsIndirect Immunofluorescence (IIF)Automated Fluorescent MicroscopyNOVA ViewDigital Imaging SystemsSlide ProcessingFluorescent MicroscopyPattern RecognitionAutomated ReadingPatient Data IntegrityPatient Safety

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