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En mus, to kulturer: isolering og dyrkning af voksne neurale stamceller fra Two Neurogene zoner Individuel Mus
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
DOI:

09:52 min

February 25, 2014

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Capitoli

  • 00:05Titolo
  • 01:41Microdissection of Mouse Brain
  • 04:13SVZ Tissue Dissociation
  • 05:43DG Tissue Dissociation
  • 07:36Cell Plating for Monolayer and Neurosphere Culture Generation
  • 08:17Results: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
  • 09:21Conclusion

Summary

Traduzione automatica

Her beskriver vi en detaljeret protokol for samtidig produktion af neurale forløber cellekulturer, som enten vedhængende monolag eller neurosfærer fra subventricular zone og dentatgyrus enkelte voksne mus.

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