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对缺血/再灌注在缺氧，饥饿型号<em> C。线虫</em
JoVE Journal
Biologia
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JoVE Journal Biologia
An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans
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对缺血/再灌注在缺氧，饥饿型号<em> C。线虫</em

DOI:
10.3791/51231-v

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09:24 min

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March 11, 2014

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, ,
1Departamento de Bioquímica, Instituto de Química,Universidade de São Paulo, 2Department of Medicine, Nephrology Division,University of Rochester Medical Center, School of Medicine and Dentistry

Capitoli

  • 00:05Titolo
  • 01:55Lab-made Hypoxic Chamber
  • 03:06Anoxia/Starvation of C. elegans
  • 05:53Identifying Dead Versus Live Worms and Performing Touch Response
  • 06:38Neuronal Modifications
  • 08:07Results: C. elegans Survival and Touch Response after Anoxia/Starvation
  • 08:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

的协议，描述了使用缺氧/饥饿在C线虫模型缺血/再灌注。功能结果包括死亡率上升，在GFP标记的神经元突起明显异常，需要神经功能受损的行为反应。

Tags

Keywords: C. ElegansAnoxiaStarvationIschemia/reperfusionNeuronal DamageTouch NeuronTouch SensitivityHypoxic IncubatorMass Flow ControlWater Bath

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