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1London Regional Cancer Program,London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry,Western University, 3Special Hematology/Flow Cytometry,London Health Sciences Centre, 4Lawson Health Research Institute, 5Department of Oncology,Western University
Capitoli
- 00:05Titolo
- 01:42Human Blood Sample Preparation and Processing
- 03:25Sample Scanning on the Analysis Instrument
- 04:58CTC Characterization for User-defined Markers Using the CSS
- 06:08Adaptation of the Standard CSS Protocol: Preclinical Mouse Models
- 07:42CTC Staining
- 09:20Scanning of Manually Separated Samples
- 10:13Results: CTC Assays in Clinical and Preclinical Research
- 13:43Conclusion
Circulerende tumorcellen (CTC's) zijn prognostische in verschillende uitgezaaide tumoren. Dit manuscript beschrijft de gouden standaard CellSearch systeem (CSS) CTC opsomming platform en hoogtepunten gemeenschappelijke misclassificatie fouten. Daarnaast worden twee aangepast protocollen beschreven gebruiker gedefinieerde marker karakterisering van CTCs en CTC opsomming in preklinische muismodellen van metastase met deze technologie.
