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展现着多药耐药<em>结核分枝杆菌</em>扩增基因芯片
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
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展现着多药耐药<em>结核分枝杆菌</em>扩增基因芯片

DOI:
10.3791/51256-v

•

07:35 min

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April 25, 2014

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, , , , , , ,
1Akonni Biosystems, Inc.

Capitoli

  • 00:05Titolo
  • 01:14Loading and Amplifying
  • 03:07Washing the Microarray
  • 04:13Imaging the Microarray
  • 05:40Artifact Detection and Microarray Sensitivity
  • 06:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

扩增微阵列结合的不对称PCR扩增和微阵列杂交到一个单一的腔室，其显著简化了微阵列的工作流程最终用户。简化工作流程芯片是一个用于创建可在低资源环境中经常使用的芯片为基础的诊断是必要的第一步。

Tags

Keywords: Multi-drug Resistant Mycobacterium TuberculosisMDR-TBAmplification MicroarrayAsymmetric PCRTarget Size SelectionTarget LabelingMicroarray HybridizationMicrofluidicBatch ProcessingGenotypingOn-chip WashClosed Amplicon MethodMultiplexingLimits Of DetectionMicroarray-based DiagnosticsClinical Practice

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