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相关的mRNA分离与酵母线粒体研究本地化翻译的机制

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

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相关的mRNA分离与酵母线粒体研究本地化翻译的机制

DOI:

10.3791/51265-v

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14:44 min

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March 14, 2014

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Chen Lesnik, Yoav Arava

¹Department of Biology,Technion – Israel Institute of Technology

Capitoli

00:05Titolo
01:05Mitochondria Purification
06:36RNA Extraction
07:59Preparing RNA for Microarray Analysis
10:04Results: Isolation of Mitochondria-associated mRNA by Subcellular Fractionation
14:13Conclusion

Summary

Traduzione automatica

编码线粒体蛋白质多的mRNA与线粒体外膜有关。我们描述了亚细胞分离过程，旨在酵母线粒体与它相关的基因和核糖体的隔离。这个协议可以被应用到不同的条件下生长的细胞，以揭示mRNA定位的机制，并靠近线粒体本地化的翻译。

Tags

MRNA Localization Mitochondrial Proteins Localized Translation Subcellular Fractionation Differential Centrifugation Yeast Model Organism Transcriptomics Proteomics

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