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从海马CA1区片核富含分的分离，研究突触核信使蛋白质的活性依赖进口核

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

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从海马CA1区片核富含分的分离，研究突触核信使蛋白质的活性依赖进口核

DOI:

10.3791/51310-v

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10:03 min

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August 10, 2014

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Pingan Yuanxiang, Sujoy Bera, Anna Karpova, Michael R. Kreutz, Marina Mikhaylova²

¹RG Neuroplasticity,Leibniz Institute for Neurobiology, ²Department of Cell Biology,Utrecht University

Capitoli

00:05Titolo
02:03Preparation of Hippocampal Slices
03:13Baseline Recording and LTP Induction
05:17Isolation of Nuclear Enriched Fractions from Hippocampal CA1
07:29Semiquantitative Immunoblotting for Synapto-nuclear Proteins
08:02Results: Induction of CA1 LTP Leads to the Accumulation of pJacS180 in the Nucleus
08:53Conclusion

Summary

Traduzione automatica

我们提供了用于诱导的长时程增强，在海马CA1区和核富集级分的从片的tetanized区域的随后分离的详细协议。这种方法可以被用来确定活性依赖的核蛋白质导入在学习和记忆的细胞模型。

Tags

Keywords: Nuclear Enriched Fraction Hippocampal Slices Synaptic Plasticity Long-term Potentiation Long-term Depression Protein Translocation Immunoblotting Activity-dependent Signaling Cascades CA1 Neurons ERK1/2 Jacob

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