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快速，特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定
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Biologia
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JoVE Journal Biologia
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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快速，特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定

DOI:
10.3791/51322-v

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11:56 min

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April 11, 2014

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,
1Molecular Pharmacology and Pathology Program, Department of Pathology & Bosch Institute,University of Sydney, 2Department of Biochemistry and Molecular Biology, School of Biomedical Sciences,Monash University

Capitoli

  • 00:05Titolo
  • 02:01Determine Intracellular Ascorbate in Cultured Cells
  • 04:16Determine the Amount of Ascorbate Released and Extracellular Ascorbate
  • 06:25Results: Determination of Intracellular and Extracellular Ascorbate in Cultured Cells
  • 11:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

抗坏血酸发挥许多重要作用的细胞代谢，其中许多只明朗化的趋势。这里，我们描述一个介质通量，为在细胞培养物内和细胞外抗坏血酸的确定具体且廉价微孔板检测。

Tags

AscorbateVitamin CMicroplate AssayIntracellularExtracellularCell CultureMedium-throughputHigh-sensitivityIron MetabolismNeuroprotectiveNeuromodulatoryBrain Homeostasis

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