Rivista
/
Biologia
/
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

DOI:
10.3791/51323-v

•

09:06 min

•

August 27, 2014

•

,
1New Jersey Neuroscience Institute,JFK Medical Center, 2Department of Biomedical Engineering,Rutgers University, 3Department of Mechanical and Aerospace Engineering,Rutgers University

Capitoli

  • 00:05Titolo
  • 01:46Preparation of DNA Gels
  • 05:34Gel Immobilization on Glass
  • 06:34Gel Functionalization
  • 07:34Results: Analysis of Stiffening and Softening DNA-crosslinked Polyacrylamide Hydrogels
  • 08:33Conclusion

Summary

Traduzione automatica

Traduzione automatica

Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.

Tags

DNA-crosslinked Polyacrylamide HydrogelsMechanobiologyDynamic StiffnessDNA CrosslinksPolyacrylamide BackboneReversible ControlGel ElasticityFibroblastNeuron Behavior

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/51323?article_type=v

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
check_url/it/51323?article_type=v

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
check_url/it/51323?article_type=v

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
check_url/it/51323?article_type=v

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
check_url/it/51323?article_type=v

FRET Imaging in Three-dimensional Hydrogels
check_url/it/51323?article_type=v

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation
check_url/it/51323?article_type=v

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
check_url/it/51323?article_type=v

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates
check_url/it/51323?article_type=v

A Novel Approach for Embedding Cell-Free Protein Synthesis Reactions in Hydrogels (Video) | JoVE
check_url/it/51323?article_type=v

Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration (Video) | JoVE

Read Article