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Fremstilling af DNA-tværbundet polyacrylamid Hydrogeler
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Biologia
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JoVE Journal Biologia
Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Fremstilling af DNA-tværbundet polyacrylamid Hydrogeler

DOI:
10.3791/51323-v

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09:06 min

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August 27, 2014

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,
1New Jersey Neuroscience Institute,JFK Medical Center, 2Department of Biomedical Engineering,Rutgers University, 3Department of Mechanical and Aerospace Engineering,Rutgers University

Capitoli

  • 00:05Titolo
  • 01:46Preparation of DNA Gels
  • 05:34Gel Immobilization on Glass
  • 06:34Gel Functionalization
  • 07:34Results: Analysis of Stiffening and Softening DNA-crosslinked Polyacrylamide Hydrogels
  • 08:33Conclusion

Summary

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Vores laboratorium har udviklet DNA-tværbundet polyacrylamid hydrogeler, et dynamisk hydrogel-system, for bedre at forstå virkningerne af modulerende væv stivhed på celle funktion. Her giver vi skemaer, beskrivelser og protokoller for at forberede disse hydrogeler.

Tags

DNA-crosslinked Polyacrylamide HydrogelsMechanobiologyDynamic StiffnessDNA CrosslinksPolyacrylamide BackboneReversible ControlGel ElasticityFibroblastNeuron Behavior

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