/

/

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

DOI:

10.3791/51342-v

•

08:37 min

•

May 05, 2014

•

Claire Chardès*¹, Pauline Mélénec*¹, Vincent Bertrand, Pierre-François Lenne

¹Institut de Biologie du Développement de Marseille,UMR7288 CNRS, Aix-Marseille Université

Capitoli

00:05Titolo
01:21Light Sheet and Detection Path Set Up
03:24Mounting C. elegans Embryos
05:28Recording C. elegans Development
07:11Multi-channel Time-lapse Z-stack Imaging of C. elegans Embryonic Development
08:10Conclusion

Summary

Traduzione automatica

This protocol describes the setup of a light sheet microscope and its implementation for in vivo imaging of C. elegans embryos.

Tags

Keywords: Light Sheet Microscopy In Toto Imaging C. Elegans Embryo Development Low Phototoxicity 3D Time-lapse Upright Microscope Opto-mechanical Setup Observation Chamber

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Generation of Stable Transgenic C. elegans Using Microinjection

Major Components of the Light Microscope

Generation of Transgenic C. elegans by Biolistic Transformation

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

A Method for Culturing Embryonic C. elegans Cells

Measurements of Physiological Stress Responses in C. Elegans

Measuring RAN Peptide Toxicity in C. elegans

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos

Read Article