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Opsætning af en Simple Light Sheet mikroskop for I Toto Imaging af C. elegans Udvikling

JoVE Journal
Biologia

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JoVE Journal Biologia

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Opsætning af en Simple Light Sheet mikroskop for I Toto Imaging af C. elegans Udvikling

DOI:

10.3791/51342-v

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08:37 min

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May 05, 2014

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Claire Chardès*¹, Pauline Mélénec*¹, Vincent Bertrand, Pierre-François Lenne

¹Institut de Biologie du Développement de Marseille,UMR7288 CNRS, Aix-Marseille Université

Capitoli

00:05Titolo
01:21Light Sheet and Detection Path Set Up
03:24Mounting C. elegans Embryos
05:28Recording C. elegans Development
07:11Multi-channel Time-lapse Z-stack Imaging of C. elegans Embryonic Development
08:10Conclusion

Summary

Traduzione automatica

Denne protokol beskriver opsætningen af en lys plade mikroskop og dens gennemførelse til in vivo billeddannelse af C. elegans embryoner.

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