È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:43Gentamicin-induced Hair Cell Necrosis and Subsequent Recovery Incubation
- 02:40Vital Dye Staining of Fish for Fluorescent Analysis of Regenerating Neuromasts
- 03:30Anesthetizing Fish and Counting Fluorescent Neuromasts
- 04:36High Resolution Fluorescent Counting of Individual Hair Cells
- 06:01Results: Lateral Line Regenerations Assay
- 09:02Conclusion
Because many zebrafish models of neurological and non-neurological diseases are studied in the adult fish rather than the embryo/larvae, we developed a quantitative lateral line regenerative assay that can be applied to adult zebrafish disease models. The assay involved resolution at the 1) neuromast and 2) individual hair cell levels.
