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Cell Labeling and Injection in Developing Embryonic Mouse Hearts
JoVE Journal
Biologia
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JoVE Journal Biologia
Cell Labeling and Injection in Developing Embryonic Mouse Hearts
DOI:

07:20 min

April 17, 2014

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Capitoli

  • 00:05Titolo
  • 01:49Collection of E9.5 and E10.5 Embryos for Ex vivo Injection
  • 03:10Ex vivo DNA or Cell Injection Under a Stereomicroscope
  • 04:35Results: Embryonic Heart Cell Labeling and Injection
  • 06:30Conclusion

Summary

Traduzione automatica

We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.

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