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Journal / Biology / Sample Preparation for Single Virion Atomic Force …
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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
 

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

Article DOI: 10.3791/51366-v • 05:31 min • January 2nd, 2014
January 2nd, 2014 •
1,2, 1,2
1Department of Physics & Astronomy, University of Utah, 2Center for Cell and Genome, University of Utah

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The attachment of virions to a surface is a requirement for single virion imaging by Super-resolution fluorescence imaging or atomic force microscopy (AFM). Here we demonstrate a sample preparation method for controlled adhesion of virions to glass surfaces suitable for use in AFM and super-resolution fluorescence imaging.

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Keywords: Sample Preparation Single Virion Atomic Force Microscopy Super-resolution Fluorescence Imaging Immobilization Glass Surface PLL-g-PEG PLL-g-PEG-Biotin Avidin Biotinylated Antibodies Virus-specific
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