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Super-resolution Imaging of Neuronal Dense-core Vesicles
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Neuroscienze
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JoVE Journal Neuroscienze
Super-resolution Imaging of Neuronal Dense-core Vesicles

Super-resolution Imaging of Neuronal Dense-core Vesicles

DOI:
10.3791/51394-v

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09:30 min

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July 02, 2014

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, , ,
1Department of Physics,Lewis & Clark College, 2Program in Biochemistry and Molecular Biology,Lewis & Clark College, 3Jungers Center for Neuroscience Research,Oregon Health & Science University, 4Department of Chemistry,Lewis & Clark College

Capitoli

  • 00:05Titolo
  • 01:12Image Acquisition
  • 05:36Image Processing, Display, and Analysis
  • 07:07Results: Representative PALM Images
  • 09:04Conclusion

Summary

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We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

Tags

Keywords: Super-resolution ImagingNeuronal Dense-core VesiclesFluorescence MicroscopyPALMPhotoactivated Localization MicroscopyVesicle CargoHippocampal NeuronsDCVDense-core VesiclesExtrasynaptic Trafficking

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