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1Genetics and Genomic Sciences Graduate Program,University of Alabama at Birmingham, 2Birmingham Veterans Affairs Medical Center, 3Department of Pathology,University of Alabama at Birmingham, 4Department of Biomedical Engineering,University of Alabama at Birmingham, 5Department of Medicine,University of Alabama at Birmingham
Capitoli
- 00:05Titolo
- 01:50HUVEC Layer Preparation
- 03:07Purified Ligand Coating
- 05:11Neutrophil Separation
- 08:33Flow Chamber Adhesion Assay
- 10:20Results: The Flow Chamber Assay Enables Analyses of Adhesive Properties of Human Peripheral Blood Neutrophils to HUVECs and Purified Ligand Substrates
- 11:49Conclusion
En metode til kvantificering af neutrofiladhæsion rapporteret. Denne metode skaber en dynamisk flow miljø svarende til den, der forekommer i et blodkar. Det giver mulighed for undersøgelse af neutrofil adhæsion til enten oprensede adhæsionsmolekyler (ligand) eller endotelcelle substrat (HUVEC) i en sammenhæng, der svarer til in vivo-miljø med sheer stress.
