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Affinity basert Isolering av Tagged Nuclei fra<em> Drosophila</em> Vev for Gene Expression Analysis
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Biologia
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JoVE Journal Biologia
Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

Affinity basert Isolering av Tagged Nuclei fra<em> Drosophila</em> Vev for Gene Expression Analysis

DOI:
10.3791/51418-v

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12:48 min

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March 25, 2014

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,
1Department of Biochemistry,Purdue University

Capitoli

  • 00:05Titolo
  • 01:30Preparation of Antibody Coated Magnetic Beads and Extraction of Nuclei from Drosophila Larval Tissues
  • 04:16Collection of Pre-isolation Samples
  • 06:16Isolation of GFP-tagged Nuclei from Drosophila Larval Tissues
  • 08:45Results: Analysis of Tagged-nuclei Enrichment “Pre-isolatoin” and “Post-isolation” Samples
  • 12:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

Drosophila vev inneholder ofte en heterogen blanding av celletyper. For å undersøke genekspresjon i spesifikke celletyper fra et bestemt vev, kan kjerner bli genetisk kodet og deretter isolert ved hjelp av en affinitets-baserte metode. Isolerte cellekjerner kan brukes til nedstrøms applikasjoner som genekspresjonsanalyse og kromatin immunoutfelling.

Tags

Affinity-based IsolationTagged NucleiDrosophilaGene Expression AnalysisCell Type-specificCentral Nervous SystemEGFPGal4/UASMagnetic BeadsTranscriptional ProfilingChromatin ImmunoprecipitationFluorescence-activated Cell SortingLaser Microdissection

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