Rivista
/
/
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
JoVE Journal
Chimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Chimica
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
DOI:

11:08 min

March 13, 2014

, , , ,

Capitoli

  • 00:05Titolo
  • 01:28Preparation of a Total Lipid Extract
  • 03:00Separation of Lipid Classes by Solid Phase Extraction
  • 05:19Preparation of Fatty Acid Methyl Esters from Cholesteryl Esters
  • 06:26Removal of Cholesterol Contamination and Gas Chromatographic Analysis
  • 08:02Results: Identification and Quantification of Fatty Acids in Rat Liver Tissue by Gas Chromatography
  • 10:24Conclusion

Summary

Traduzione automatica

Pregnancy leads to significant changes to the fatty acid composition of maternal tissues. Lipid profiles can be obtained via gas chromatography to allow identification and quantification of fatty acids in individual lipid classes among rats fed various high and low fat diets during pregnancy.

Video correlati

Read Article