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使用气相色谱怀孕期间分析脂肪酸组成的变化在大鼠肝组织
JoVE Journal
Chimica
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JoVE Journal Chimica
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
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使用气相色谱怀孕期间分析脂肪酸组成的变化在大鼠肝组织

DOI:
10.3791/51445-v

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11:08 min

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March 13, 2014

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, , , ,
1Human Development & Health Academic Unit, Faculty of Medicine,University of Southampton

Capitoli

  • 00:05Titolo
  • 01:28Preparation of a Total Lipid Extract
  • 03:00Separation of Lipid Classes by Solid Phase Extraction
  • 05:19Preparation of Fatty Acid Methyl Esters from Cholesteryl Esters
  • 06:26Removal of Cholesterol Contamination and Gas Chromatographic Analysis
  • 08:02Results: Identification and Quantification of Fatty Acids in Rat Liver Tissue by Gas Chromatography
  • 10:24Conclusion

Summary

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Traduzione automatica

妊娠导致显著改变母体组织中的脂肪酸组合物。血脂可通过气相色谱法来获得允许在怀孕期间大鼠中识别和个别类脂质的脂肪酸定量饲喂各种高，低脂肪饮食。

Tags

Gas ChromatographyFatty AcidsLipid AnalysisRat LiverPregnancySolid Phase ExtractionTriacylglycerolsPhospholipidsCholesteryl EstersOmega-3Omega-6

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