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Brug af gaskromatografi at analysere kompositoriske Ændringer af fedtsyrer i rottelevervæv under graviditet
JoVE Journal
Chimica
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JoVE Journal Chimica
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
DOI:

11:08 min

March 13, 2014

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Capitoli

  • 00:05Titolo
  • 01:28Preparation of a Total Lipid Extract
  • 03:00Separation of Lipid Classes by Solid Phase Extraction
  • 05:19Preparation of Fatty Acid Methyl Esters from Cholesteryl Esters
  • 06:26Removal of Cholesterol Contamination and Gas Chromatographic Analysis
  • 08:02Results: Identification and Quantification of Fatty Acids in Rat Liver Tissue by Gas Chromatography
  • 10:24Conclusion

Summary

Traduzione automatica

Graviditet medfører væsentlige ændringer i fedtsyresammensætningen af ​​maternelle væv. Lipidprofiler kan hentes via gaskromatografi for at muliggøre identifikation og kvantificering af fedtsyrer i enkelte lipidklasser blandt rotter fodret forskellige high og fedtfattig kost under graviditeten.

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