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High Throughput Kvantitativ Expression Screening og oprensning Anvendt til rekombinant disulfid-rige Venom Proteiner fremstillet i<em> E. coli</em
JoVE Journal
Biologia
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JoVE Journal Biologia
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

High Throughput Kvantitativ Expression Screening og oprensning Anvendt til rekombinant disulfid-rige Venom Proteiner fremstillet i<em> E. coli</em

DOI:
10.3791/51464-v

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12:16 min

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July 30, 2014

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, , ,
1Architecture et Fonction des Macromolécules Biologiques (AFMB),Aix-Marseille Université, 2iBiTec-S, Service d’Ingénierie Moléculaire des Protéines (SIMOPRO),Commissariat à l’énergie atomique et aux énergies alternatives (CEA) Saclay, France

Capitoli

  • 00:05Titolo
  • 01:27Target Protein Test Expression
  • 05:32Purification and Analysis
  • 09:22Results: Representative Expression Screen of 96 Disulfide-rich Venom Proteins
  • 11:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

En protokol til kvantitativ, high throughput udtryk screening og analytisk oprensning af fusionsproteiner fra små Escherichia coli kulturer er beskrevet og anvendt til at udtrykke disulfid-rigt dyre gift protein-targets.

Tags

Keywords: High ThroughputQuantitative Expression ScreeningRecombinant Disulfide-rich Venom ProteinsE. ColiStructural GenomicsDsbCDisulfide Bond FormationProtein ProductionTherapeutic Drug LeadsVenom ProteinsMass SpectrometryMicro-assays

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