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In-vitro-Methode zur E-Selektin-vermittelte Interaktionen zwischen Prostata zirkulierenden Tumorzellen, abgeleitet von Patienten und humanen Endothelzellen beachten
JoVE Journal
Medicina
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JoVE Journal Medicina
In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
DOI:

11:56 min

May 15, 2014

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Capitoli

  • 00:05Titolo
  • 01:50Culturing HUVECs on Microslides for Observing Circulating Tumor Cell (CTC)-endothelial Interactions
  • 04:04Preparation of Flow Chamber Assembly for Overnight HUVEC Culture on a Microslide
  • 05:48Preparation of Anti-PSMA Labeled Prostate Cancer Cells
  • 07:05Preparation of Microscope, Syringe Pump and Flow Chamber Assembly
  • 09:11Results: Analysis of Interactions Between Labeled MDA Prostate Cancer Cells and Endothelial Cells
  • 11:11Conclusion

Summary

Traduzione automatica

Unser Bericht beschreibt eine einzigartige Methode zur Visualisierung und Analyse von CTC / EG Wechselwirkungen bei Prostatakrebs unter physiologischen Flussbedingungen.

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