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JoVE Journal Neuroscience
In vivo Optogenetic Stimulation of the Rodent Central Nervous System
 

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Article DOI: 10.3791/51483-v • 09:37 min • January 15th, 2015
January 15th, 2015 •
1, 2, 3, 4, 2,5, 1
1Department of Psychiatry, University of Pittsburgh Medical Center, 2Department of Bioengineering, Stanford University, 3Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, 4Department of Neurobiology and Behavior, Cornell University, 5Department of Psychiatry and Behavioral Sciences, Stanford University

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Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.

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In Vivo Optogenetic Stimulation Rodent Central Nervous System Neural Circuits Awake Animals Freely-moving Animals Cell-type Specificity Spatial Precision High Temporal Resolution Neuroscientists Complex Behavioral States Optogenetics Neuroscience Causal Testing Neural Signaling Events Behavior Optical And Genetic Approaches Light-sensitive Ion Channels Opsins Mammalian Cells Specific Wavelengths Of Light Deep Brain Structures Awake Rodents Neural Circuit Modulation Theoretical Principles Of Light Transmission Experimental Consideration Laser Configurations Tethering Strategies Simultaneous Stimulation High-throughput Behavioral Testing
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