Rivista
/
Chimica
/
Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
JoVE Journal
Chimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Chimica
Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

DOI:
10.3791/51487-v

•

13:00 min

•

April 04, 2014

•

, ,
1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology,University of Bologna

Capitoli

  • 00:05Titolo
  • 02:01Preparing Samples and Performing the Experiment
  • 06:47Data Analysis
  • 09:24Results: Quantification of Enzyme Catalysis by Isothermal Titration Calorimetry
  • 12:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.

Tags

Isothermal Titration CalorimetryITCEnzymatic ReactionsEnzyme KineticsThermodynamicsKcatKMEnthalpyUreaseJack Bean

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis

Retropinacol/Cross-pinacol Coupling Reactions – A Catalytic Access to 1,2-Unsymmetrical Diols

Mizoroki-Heck Cross-coupling Reactions Catalyzed by Dichloro{bis[1,1′,1”-(phosphinetriyl)tripiperidine]}palladium Under Mild Reaction Conditions

Light-driven Enzymatic Decarboxylation

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

A Protocol for Safe Lithiation Reactions Using Organolithium Reagents

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine

Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory

Imine Metathesis by Silica-Supported Catalysts Using the Methodology of Surface Organometallic Chemistry

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids

Read Article