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生物膜的微阵列用刮墨刀型组合方式形成

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Bioingegneria

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JoVE Journal Bioingegneria

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

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生物膜的微阵列用刮墨刀型组合方式形成

DOI:

10.3791/51501-v

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07:56 min

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May 08, 2014

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Nathan J. Wittenberg, Timothy W. Johnson, Luke R. Jordan, Xiaohua Xu, Arthur E. Warrington, Moses Rodriguez⁴, Sang-Hyun Oh²

¹Department of Electrical and Computer Engineering,University of Minnesota, ²Department of Biomedical Engineering,University of Minnesota, ³Department of Neurology,Mayo Clinic College of Medicine, ⁴Department of Immunology,Mayo Clinic College of Medicine

Capitoli

00:05Titolo
01:27Preparation of Vesicles
02:30Formation of Spherical Supported Lipid Bilayers (SSLB)
03:43Assembling the SSLB Array
04:32Cholera Toxin Binding Assay
05:31Typical Microwell Intensities and Array Applications
07:12Conclusion

Summary

Traduzione automatica

支持的脂质双层和天然膜颗粒是方便的系统，可以近似细胞膜的性质和在各种分析战略中。这里，我们证明了制备微阵列支持的脂质双层包被的SiO ₂的有孔玻璃珠，磷脂囊泡或天然膜颗粒组成的方法。

Tags

Biomembrane Microarray Supported Lipid Bilayers Spherical Supported Lipid Bilayers Squeegee-based Assembly Microfabricated Microwells Cholera Toxin GM1 Ganglioside Lipid Vesicles Cellular Biomembranes

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