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体外聚集测定使用tau蛋白的蛋白质
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Biologia
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JoVE Journal Biologia
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
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体外聚集测定使用tau蛋白的蛋白质

DOI:
10.3791/51537-v

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09:22 min

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January 02, 2015

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, ,
1Department of Biochemistry and Molecular Biology,Michigan State University

Capitoli

  • 00:05Titolo
  • 01:19Preparations
  • 02:14Terminal Assay with No Dye
  • 04:44Continual Mode Assay Using a Plate Reader With Dye
  • 06:14Continual Mode Assay Using a Compact Spectrofluorometer With Dye
  • 07:33Results: Tau Aggregation Assayed by Different Modes and Instruments
  • 08:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

未修饰的和磷酸化的tau蛋白以两种体外聚集测定中使用以显示磷酸化依赖快速聚合动力学。这些测定铺路用于可调节tau蛋白的倾向，以形成背后阿尔茨海默氏病的进展纤丝化合物未来画面。

Tags

Alzheimer’s DiseaseTauopathiesHyperphosphorylated TauNeurofibrillary TanglesTau Aggregation AssaysRecombinant TauHigh-throughput ScreensTau AggregatesNeurodegenerative DisordersTherapeutic Development

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