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Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
JoVE Journal
Biologia
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JoVE Journal Biologia
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

DOI:
10.3791/51583-v

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07:35 min

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June 09, 2014

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, ,
1Department of Biomedical Engineering,Duke University, 2Research Triangle MRSEC,Duke University

Capitoli

  • 00:05Titolo
  • 01:37Expression of Elastin-like Polypeptides (ELPs)
  • 02:22Purification of ELPs by Inverse Transition Cycling
  • 04:17Removal of Contaminants from ELP through Subsequent Rounds of ITC
  • 05:22Characterization of ELP Tt by Temperature-programmed Turbidimetry
  • 06:16Results: Characterization of ELPs Purified from E. coli through ITC
  • 07:13Conclusion

Summary

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Traduzione automatica

Elastin-like polypeptides are stimulus-responsive biopolymers with applications ranging from recombinant protein purification to drug delivery. This protocol describes the purification and characterization of elastin-like polypeptides and their peptide or protein fusions from Escherichia coli using their lower critical solution temperature phase transition behavior as a simple alternative to chromatography.

Tags

Keywords: Elastin-like PolypeptidesRecombinantNon-chromatographic PurificationLower Critical Solution TemperaturePhase TransitionCoacervationEscherichia ColiPeptide FusionProtein FusionBiosensingDrug DeliveryThermal Properties

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