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Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva
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Biologia
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JoVE Journal Biologia
Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

DOI:
10.3791/51595-v

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08:22 min

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June 17, 2014

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,
1DFG-Center for Regenerative Therapies Dresden,Technische Universität Dresden

Capitoli

  • 00:05Titolo
  • 01:20Preparation of Glass Needles and Morpholino Solution
  • 02:26Larval Injections and Analysis
  • 05:14Assessment of Regenerative Outgrowth
  • 06:21Results: Transfection Using Cardiac Ventricular Injection
  • 07:52Conclusion

Summary

Traduzione automatica

Traduzione automatica

An adaptable reverse genetic method for zebrafish to assess gene function during later stages of development and physiological homeostasis such as tissue regeneration using intraventricular injections of gene-specific morpholinos.

Tags

Keywords: ZebrafishReverse GeneticsMorpholinoCardiac Ventricular InjectionGene FunctionEmbryogenesisFinfold RegenerationVertebrate Regeneration

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