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Capitoli
- 00:05Titolo
- 01:07Preparation of Setup
- 02:06Perform Redox Titration
- 03:12Measure Titration Samples Using EPR Spectroscopy and Data Analysis
- 04:12Results: Identification of Redox Active Cofactors of S. cerevisiae Nar1 and Their Midpoint Potentials
- 05:33Conclusions
The goal of this protocol is to use electron paramagnetic resonance (EPR) monitored redox titrations to identify different cofactors of Saccharomyces cerevisiae Nar1. Redox titrations offer a very robust way to obtain midpoint potentials of different redox active cofactors in enzymes and proteins.
