Rivista
/
Immunologia e infezioni
/
Anvendelse af fluorescerende Skud Arrays for Vurdering af T-celle respons<em> In vivo</em
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

Anvendelse af fluorescerende Skud Arrays for Vurdering af T-celle respons<em> In vivo</em

DOI:
10.3791/51627-v

•

12:09 min

•

June 19, 2014

•

, , ,
1Department of Immunology, John Curtin School of Medical Research,Australian National University

Capitoli

  • 00:05Titolo
  • 01:39Fluorescent Target Array Preparation: CTV and CFSE Labeling
  • 04:54FTA Preparation: Peptide Pulsing and Cell Washing
  • 07:50FTA Preparation: CPD Cell Labeling
  • 09:43FTA Assay in Response to VV/HIV Epitopes
  • 11:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

Muligheden for at overvåge T-cellereaktioner i detaljer in vivo er vigtig for udviklingen af vores forståelse af immunresponset. Her beskriver vi brug af fluorescerende target arrays (FTA) i en in vivo T-celle analyse, der vurderer> 250 parametre samtidigt ved flowcytometri.

Tags

Fluorescent Target ArrayFTACFSECTVCPDMHC Class IMHC Class IICD8+ T CellsCD4+ T CellsT Cell ResponseIn VivoFlow CytometryAntigen EpitopesRecombinant Pox Virus Vaccine

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Introducing Shear Stress in the Study of Bacterial Adhesion

Induktion af graft-versus-host sygdom og<em> In Vivo</em> T Cell Monitoring Brug en MHC-matchet murin model

Transplantation af Tail Hud til Study Allogen CD4 T-celle respons i mus

In Vitro og In Vivo evaluering af T, B og myeloide celler smittespredningshæmmende aktivitet og humorale svar fra transplanterede

In Vivo Assay til påvisning af Antigen-specifikke T-celle cytolytisk funktion ved hjælp af en Vaccination Model

Isolering af Salmonella typhimurium-indeholdende Phagosomes fra makrofager

En suge Blister protokol at studere Human T-celle hjemkalde svar In Vivo

Evaluering af Zika Virus-specifikke T-celle respons i Immunoprivileged organer af inficerede Ifnar1- / - mus

Read Article