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Immunology and Infection

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JoVE Journal Immunology and Infection
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
 

Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

Article DOI: 10.3791/51643-v • 08:04 min • July 9th, 2014
July 9th, 2014 •
1, 1, 1, 1, 2,3, 1,3
1Unité d'Immunopathologie Virale, Centre de Recherche du CHU Sainte-Justine, Department of Microbiology, Infectiology & Immunology, Faculty of Medicine, Université de Montréal, 2Infectious Diseases Service, CHU Sainte-Justine, Faculty of Medicine, Université de Montréal, 3Department of Paediatrics, Université de Montréal

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Novel generations of functional assays such as gamma interferon (IFN-γ) ELISpot, which detect cytokine production at the single cell level and provide both quantitative and qualitative characterization of T cell responses can be used to assess cell-mediated immune responses directed against varicella zoster virus (VZV).

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Keywords: IFN-γ ELISpot Assay Varicella-zoster Virus Cell-mediated Immunity Umbilical Cord Blood Transplantation VZV Prophylaxis T Cell Reconstitution VZV-specific T Cell Response
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