Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Using Fluorescent Proteins to Monitor Glycosome…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
 

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Article DOI: 10.3791/51647-v • 10:04 min • August 19th, 2014
August 19th, 2014 •
1, 1, 1
1Department of Genetics and Biochemistry, Clemson University Eukaryotic Pathogens Innovation Center

Capitoli

Riepilogo
Automatic Translation

Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.

Tags

Keywords: Fluorescent Proteins Glycosome Dynamics Trypanosoma Brucei Human African Trypanosomiasis Nagana Peroxisomes Glycolysis Fluorescence Microscopy PTS2-eYFP Flow Cytometry Organelle Composition
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter