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测量渗透水渗透系数（P<sub> F</sub>）球形细胞：隔离植物原生质体为例。
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Biologia
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JoVE Journal Biologia
Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
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测量渗透水渗透系数（P<sub> F</sub>）球形细胞：隔离植物原生质体为例。

DOI:
10.3791/51652-v

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14:20 min

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October 08, 2014

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, , , , ,
1The RH Smith Institute of Plant Sciences and Genetics in Agriculture,The Hebrew University of Jerusalem, 2Earth and Life Institute,Université catholique de Louvain, 3Institut des Sciences de la Vie,Université catholique de Louvain

Capitoli

  • 00:05Titolo
  • 02:10Isolation of Arabidopsis Mesophyll Protoplasts
  • 04:13The Hypotonic Challenge Assay: Arabidopsis Mesophyll Cell Swelling
  • 06:36Analysis of Cell Volume Change Using ImageJ
  • 08:12Modling the Rate of Osmolarity Change in the Experimental Chamber Using ImageJ and the Matlab Program PfFit
  • 11:11Determining the Pf Using the Matlab Fitting Program PfFit
  • 12:30Results: Determination of the Osmotic Water Permeability Coefficient (Pf) in Plant Protoplasts
  • 13:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

测量细胞的渗透水的渗透系数（P F）可以帮助理解水通道蛋白的调节机制（水通道蛋白）。在这里介绍的球形植物细胞原生质体P F决心涉及原生质体的分离和体积变化的过程中不断的浴灌注渗透挑战，因此他们的初始速度的数值分析。

Tags

Osmotic Water PermeabilityPlant ProtoplastsAQP RegulationCell Volume ChangesHypotonic SolutionOsmotic Challenge AssayPf Measurement

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